EGF R Antibody orb1252778 from Biorbyt

Product Specs
ItemEGF R Antibody
CompanyBiorbyt
Price Pricing Info Supplier Page
Catalog Numberorb1252778
ClonalityMonoclonal
ReactivityHuman
FormatLiquid
ApplicationsFC, IF, IHC, WB
ImmunogenThe immunizing protein for this antibody was recombinant human EGFR
TargetEGF R
IsotypeIgG2a, kappa
Conjugate/TagUnconjugated
HostMouse
Quantity100 µg
Concentration0.2 mg/mL
NCBI Full Gene Nameepidermal growth factor receptor
NCBI Gene AliasesPIG61, ERBB1, NISBD2, ERBB, HER1, ERRP, mENA
CloneGFR450
StorageAliquot and Store at 2-8°C. Avoid freez-thaw cycles

Specifications/Features

Application Notes: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Application Dilutions: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

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